Infiltrative monocyte derived adenosine deaminase 2 is associated with COVID-19 related acute respiratory distress syndrome

Abstract Acute respiratory distress syndrome (ARDS) is a severe complication of COVID-19 characterized by immune dysregulation. The pathogenesis for this, particularly the involvement infiltrative monocytes, poorly understood. Extracellular ATP released during inflammation and hypoxia rapidly scavenged into adenosine, which further converted inosine adenosine deaminase (ADA). ADA2 an extracellular isoform ADA secreted activated monocytes expressed infiltrated CD16 +CXCR3 +monocytes. Herein, we aim to study impact monocyte-derived in associated ARDS. Critically ill patients were assigned ARDS group based on Berlin criteria, while non-ARDS included without any or with low-flow oxygen therapy. We observed elevated activity serum patients, reflecting increased level protein, when compared group. exhibited high circulating IL-6, CXCL8 CXCL10. Notably, correlation analysis revealed significant association between CXCL10, chemokine CXCR3 Post-mortem bulk RNAseq lung tissues showed expression gene, CECR1, along CXCL10 suggesting intrapulmonary accumulation positive macrophages. These observations supported single-nuclear spatial transcriptomic atlas demonstrated strong In summary, our data suggest potential role modulating response related Supported German Center Infection Research (TI 07.005 STH)